The Gadsden flag, featuring a rattlesnake and the word "Don't Tread on Me

https://andersonfowi396.novacrestiq.com/posts/connecting-generations-through-history-teaching-kids-about-civil-war-flags-2

The Gadsden flag, offering a rattlesnake and the word "Don't Tread on Me," was adopted via a few Confederate items as a symbol of resistance in opposition to perceived oppression.